Paper Search
Main Menu
Homepage
Submission
Papers In Press
Search
Information for Contributors
Introducing the Journal
Board of Journal of Science
Contact address
 
Select by Volumes


 
 
 
 
 
 
 
SCImago Journal & Country Rank
 
Home > Screening of Volcanic Cave Actinomycetes for Antimicrobial Activity Against Paenibacillus larvae, A Honey Bee Pathogen
 
Screening of Volcanic Cave Actinomycetes for Antimicrobial Activity Against Paenibacillus larvae, A Honey Bee Pathogen
Paper Type
Contributed Paper
Title
Screening of Volcanic Cave Actinomycetes for Antimicrobial Activity Against Paenibacillus larvae, A Honey Bee Pathogen
Author
Silvia Kay [a], Wasu Pathom-aree [b], and Naowarat Cheeptham*[a]
Email
ncheeptham@tru.ca
Abstract:

Actinomycete strains isolated from Helmcken Falls cave in Wells Gray Provincial Park, British Columbia were discovered to have antimicrobial activity against Paenibacillus larvae, the causative agent of American foulbrood disease in honeybees.  The antimicrobial activity of 119 isolates grown in three fermentation liquid media, namely YMG, AMHU-4 and V8 juice, at 28°C on an orbital shaker at 250 rpm for 7 days, were tested against P. larvae (ATCC 13537T) using the paper disc agar diffusion method.  Of the strains screened, strain E9, grown in V-8 juice medium showed significant antibacterial activity against P. larvae with a maximum activity on day 4 of incubation at pH 7.  Chemotaxonomic study and phylogenetic analysis based on 16S rRNA gene sequence revealed that strain E9 is a member of the genus Streptomyces.  This is the first report on bioprospecting in volcanic caves of Canada.

Start & End Page
26 - 33
Received Date
2012-01-26
Accepted Date
2012-08-27
Full Text
Download
Correspondence:
Author Name
Keyword:
Keyword
actinomycetes, volcanic caves, Paenibacillus larvae, antimicrobial activity, American foulbrood disease (AFB),
Volume
Vol.40 No.1 (JANUARY 2013)